Operation: measure skeleton length

measure skeleton length operation

Author:

Volker Bäcker

Example

input image    result    Remark that the area in the example is 488 square-pixel.

Description

Measure the length of a skeleton using the algorithm from  "Robust Quantification of In Vitro Angiogenesis Through Image Analysis", published in IEEE Transactions on Medical Imaging Vol24, No.4, April 2005, as described in the ImageJ mailing list by Michael Miller. The aspect ratio of  a pixel is considered to be one. Lengths are calculated from the middle of one pixel to the middle of another pixel. Diagonal steps are by a factor of sqrt(2) longer than horizontal or vertical steps.

Options

The operation has no options.

Parameter

The only parameter is the input image. The input image must be a skeleton. The background value must be zero and the skeleton value 255.

Results

The result is a results table with the measured length. The result is either in pixel or in the defined unit, if a scale is set.